Angiotensin in Blood and Lymph following Reduction in Renal Arterial Perfusion Pressure in Dogs.

Methods Fasting adult mongrel dogs weighing 9 to IS kg were anesthetized with morphine sulfate (2 mg/kg subcutancously) and sodium pentobarbital (15 mg/kg intravenously) or with sodium pentobarbital alone (30 mg/kg intravenously). In some experiments the vagus-sympathetic-depressor trunks were cut. Intermittent positive pressure respiration was used unless otherwise stated. Arterial pressure was recorded from a femoral artery by a mercury or by a strain gauge manometer. Five per cent dextrose in saline was given by slow intravenous drip in all experiments. In 17 dogs, one kidney was removed and a Goldblatt clamp applied loosely to the contralateral renal artery. Both procedures were performed retroperitoneally through flank incisions. In S of these 17 dogs, the thoracic duct was cannulated in the neck and lymph allowed to drain externally. Renal vein blood was obtained in 10 dogs by introducing a polyethylene catheter (outside diameter 2 mm) into the renal vein on the nephreetomized side, passing it across the inferior vena cava into the eontralateral renal vein and manipulating it into the renal hiluin. Arterial blood was obtained through a polyethylene catheter from